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5-lb All-In-One Grow Bag Instructions

🧪 Step 1: Inoculation

  • Use a liquid culture (LC) or spore syringe with a sterile needle.

  • Shake your syringe to distribute spores or mycelium evenly.

  • Sanitize the injection port area and needle with 70% isopropyl alcohol.

  • Inject 5–10 mL through the self-healing injection port.

📹 Want to see it in action?
👉 Watch our 2.5-lb bag inoculation on TikTok
(Same process applies to the 5-lb bags!) 

📝 Optional but recommended: Label your bag with the strain and date of inoculation.

🌡 Step 2: Incubation

  • Store the bag in a dark, clean space at 70–78°F (21–26°C).

  • Avoid areas with large temperature swings or direct sunlight.

  • Do not open the bag—your filter patch provides gas exchange.

⏳ Colonization usually takes 2–4 weeks. You’ll see white mycelium spreading throughout the substrate.

💡 Pro Tip: Once the grain is colonized (but before full substrate colonization), you can gently break up and remix the bag to speed up total colonization. Wait until about 30–50% colonized before doing this.

📹 Want to watch us mix up a partially colonized bag?
👉 Check out this real-time colonization progress on TikTok

🍄 Step 3: Fruiting

Once the bag is fully colonized (completely white), it’s time to fruit.

You have two options:

✅ Option 1: In-Bag Fruiting (Easiest)

  1. Make a small X-cut or slit in the plastic on the side or top of the bag.
  2. Mist the inside of the bag once per day to maintain humidity.
  3. Place the bag somewhere with indirect light (natural daylight or a lamp).
  4. Maintain a room temperature of 65–75°F (18–24°C).

🍄 Option 2: Bulk Fruiting Chamber (More Yield, More Setup)

  1. Cut open the top of the bag and place the colonized block in a monotub, shoebox, or mini greenhouse with high humidity and airflow.
  2. Mist and fan as needed, or automate your setup with fans/humidifiers.
  3. Aim for 95% humidity and plenty of fresh air exchange.

    📖 Want a full walkthrough?
    👉 Check out our blog: Grow Bags to Monotubs – Step-by-Step

📆 Step 4: Harvesting

  • Mushrooms are ready to harvest when the caps begin to flatten out (just before they fully open).

  • Gently twist and pull from the base or use clean scissors.

  • Don’t wait too long—overripe mushrooms drop spores and reduce yield.

After harvest, you may get a second or even third flush. To encourage more growth:

  • Let the block rest for 2–3 days.

  • Mist heavily to rehydrate.

  • Keep humidity high and light consistent.

🛠 Troubleshooting & Tips

❌ Contamination?
If you see green, black, or pink spots—or smell something sour—it’s likely contamination. Remove the bag from your grow area and discard it.

💤 Slow Growth?
Check your temperature. Too cold = stalled growth. Too hot = contamination risk. Keep it in that 70–78°F sweet spot.

💦 Not Fruiting?
Your bag might be too dry. Increase misting or add it to a fruiting chamber.

🤝 Need Help?

We’re here to support your grow!
💬 DM us on Instagram @SeismicSpore
📩 Or email us at [email protected]

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